Purolite develops and manufactures resin beads used in the most regulated industries to separate, remove or recover very specific elements and compounds. Purolite produces more than 1,000 active commercial products.
Our healthcare and life science products provide innovative solutions for a range of applications, including chromatographic separation, diagnostics, nanoparticle milling, APIs and biocatalysis. These resins are solving critical challenges across global medical device, nutraceutical, and pharmaceutical industries. Our unmatched technical support, regulatory expertise and commitment to security of supply place Purolite resins at the forefront of the global Healthcare and Life Sciences marketplace.
Praesto® bioprocessing products aim to improve the accessibility and availability of cutting-edge antibody therapeutics worldwide, which are typically used to treat, extend life and improve quality of life for patients with cancer, arthritis, diabetes and many other conditions. These agarose-based chromatographic resins are key raw materials for the downstream processing of antibodies and recombinant proteins, and are trusted by many of the leading biopharmaceutical manufacturers and CMOs. With hand-in-hand applications support and patented manufacturing technology, Praesto resins are currently implemented in FDA-approved commercial manufacturing processes and hundreds of clinical trials across the globe.
The Praesto mAb Platform
The purification of commercially-available monoclonal antibodies (mAbs) on the market today is typically performed in three chromatography steps. Purolite offer industry-leading solutions for each step.
Protein A Affinity Chromatography
A Protein A affinity resin is utilized as an initial antibody capture step. Praesto Protein A resins deliver exceptionally high purity (>99%) and yield in a single, efficient step.
Cation Exchange Chromatography
A Sulphopropyl- (SP) functionalized cation exchange resin in ‘bind and elute’ mode is used to remove aggregates and HCP (Host Cell Proteins).
Anion Exchange Chromatography
A Quarternary Amine (Q) functionalized anion exchange resin is used in ‘flow-through’ mode to remove trace contaminates and ensure sufficient viral clearance.
Products for Protein A Chromatography
Presto® Jetted A50
Praesto Jetted A50 is a high performance agarose-based Protein A resin that delivers exceptional performance in terms of capacity, pressure flow and lifetime. Designed to meet the mAb processing demands of tomorrow, today, the combination of a highly cross-linked Praesto agarose base matrix with the NGL-ImpactTM A ligand from Repligen® provides increased capacity and excellent alkaline stability, reducing your bioburden risk and improving your process efficiencies from the same manufacturing footprint.
Praesto® Jetted A50 HipH
The unique properties of Praesto Jetted A50 HipH address challenges in eluting pH-sensitive mAbs and Fc-containing proteins, which can become unstable at pH levels typically used for elution with other Protein A resins (3 – 3.5). This resin provides alkaline stability, capacity of 60 g/L for polyclonal human IgG and elution up to pH 5.
This creates a wider operational window for your Protein A step, facilitating rapid process development and processing of all Fc-containing molecules by:
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- enabling use of a classic antibody purification platform
- retaining the unique specificity of Protein A for high recovery and excellent productivity
- minimizing impact on facility fit with comparable elution volumes and buffer usage
- lowering levels of post-Protein A contaminants, reducing polishing challenges and driving process robustness
Products for CEX Chromatography
Praesto® SP45
Agarose Highly Cross Linked Strong Cation Exchanger, 45 μm particle size
PRINCIPAL APPLICATIONS
- Protein purification (20-500 kDa)
- MAb Purification
ADVANTAGES
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Excellent dynamic binding capacities for high-productivity and scalability
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Excellent pressure/flow performance
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High resolution/selectivity
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Excellent chemical and physical stability
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Secure, validated supply and regulatory support
Praesto® SP65
Agarose Highly Cross Linked Strong Cation Exchanger, 65 μm particle size
PRINCIPAL APPLICATIONS
- Protein purification (20-500 kDa)
- MAb Purification
ADVANTAGES
-
Excellent dynamic binding capacities for high-productivity and scalability
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Excellent pressure/flow performance
-
High resolution/selectivity
-
Excellent chemical and physical stability
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Secure, validated supply and regulatory support
Praesto® SP90
Agarose Highly Cross Linked Strong Cation Exchanger, 90 μm particle size
PRINCIPAL APPLICATIONS
- Protein purification (20-500 kDa)
- MAb Purification
ADVANTAGES
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Excellent dynamic binding capacities for high-productivity and scalability
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Excellent pressure/flow performance
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High resolution/selectivity
-
Excellent chemical and physical stability
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Secure, validated supply and regulatory support
Praesto®
Jetted SP35
Agarose Highly Cross Linked Strong Cation Exchanger, Uniform Particle Size, 35 μm
PRINCIPAL APPLICATIONS
- Protein purification (20-500 kDa)
- MAb Purification
ADVANTAGES
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Manufactured using patented Jetting technique
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Uniform particle size
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Enhanced productivity
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High dynamic binding capacity
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Enhanced pressure/flow performance Manufactured with reduced environmental impact
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Secure, validated supply and regulatory support
Products for AEX Chromatography
Praesto®
Jetted Q35
Agarose Highly Cross Linked Strong Anion Exchanger, Uniform Particle Size, 35 µm
PRINCIPAL APPLICATIONS
- MAb Purification
- Protein purification (20-500 kDa)
ADVANTAGES
- Manufactured using patented Jetting technique
- Uniform particle size
- High capacity Enhanced productivity
- Enhanced pressure/flow performance
- Manufactured with reduced environmental impact
- Secure, validated supply and regulatory support
Praesto® Q45
Agarose Highly Cross Linked Strong Anion Exchanger, 45 μm particle size
PRINCIPAL APPLICATIONS
- Protein purification (20-500 kDa)
- MAb Purification
ADVANTAGES
-
Excellent dynamic binding capacities for high-productivity and scalability
-
Excellent pressure/flow performance
-
High resolution/selectivity
-
Excellent chemical and physical stability
-
Secure, validated supply and regulatory support
Praesto® Q65
Agarose Highly Cross Linked Strong Anion Exchanger, 65 μm particle size
PRINCIPAL APPLICATIONS
- Protein purification (20-500 kDa)
- MAb Purification
ADVANTAGES
-
Excellent dynamic binding capacities for high-productivity and scalability
-
Excellent pressure/flow performance
-
High resolution/selectivity
-
Excellent chemical and physical stability
-
Secure, validated supply and regulatory support
Praesto® Q90
Agarose Highly Cross Linked Strong Anion Exchanger, 90 μm particle size
PRINCIPAL APPLICATIONS
- Protein purification (20-500 kDa)
- MAb Purification
ADVANTAGES
-
Excellent dynamic binding capacities for high-productivity and scalability
-
Excellent pressure/flow performance
-
High resolution/selectivity
-
Excellent chemical and physical stability
-
Secure, validated supply and regulatory support