NXTchrom is a range of configurable chromatography systems designed to meet all chromatography-based process requirements and the design can handle any column diameter. We use a proven platform that allows you to integrate different options into one core platform.
With a self-contained design, the NXTchrom System incorporates pumps, valves, and instruments in a single system. The system’s control cabinets include pneumatic and electric components, and the system can be controlled using an easy-to-use touch screen interface.

The NXTchrom system is housed on a sturdy stainless steel frame with a closed construction design. The Stainless steel frame is also used to lift the system for easy connection to the plant drain, the electrical cabinet is installed on the SS frame along with the touchscreen for making the system easy to use for the operator.

Modular Concept

The platform design allows easy customization of the process chromatography system. One or more systems that meet your precise process requirements can be produced by choosing from the required options. You can choose from a variety of sensors and specify the number of valves and ports you need.

Options to choose from:

    • Additional inlet and outlet valves
    • Inline filter housing (1 or 2)
    • Pressure sensor (before filter or after column)
    • Air sensor (after bubble trap, before pump, or both)
    • Additional conductivity and temperature sensors before column)
    • Additional pH sensor (before column)
    • Sampling valve (after column)
    • Position detectors for valves
    • Steam-in-place (SIP)
    • Clean-in-place (CIP)
    • Gradient function (controlled either by flow rate only or by flow rate and conductivity)

Each option includes the required combination of hardware and software modules. All standard features and options have been validated on the platform.

User-Friendly Software:

The control unit uses a simple, user-friendly interface via the touchscreen for data input and programming commands. The system is shielded from unauthorized access with password protection (four levels), and all events and actions (alarms, process steps, manual commands, etc.) are recorded in accordance with cGMP guidelines. The program allows you to operate the system manually or automatically.

The automatic mode has:

    • Multiple operations
    • Configurable fluid paths
    • Control of inlets/outlets and column valve
    • Multiple end conditions
    • Pause and hold alarms
    • Interactive pausing steps
    • Full trend review, trend analysis, and printing from the system are all standard. Data export and configurable interfacing to external software is also included.

Hygienic Design:

All process piping and valves are made from Stainless Steel 316L (ASTM 1.4404) to conform to ASME BPE requirements. Only membrane valves have been used for the process flow path. Block body valves, compact instruments, and other devices have been used to minimize the holdup volumes. Cables and pneumatic lines are run through stainless steel conduits protecting them from damage or contamination.

The NXTchrom system can be cleaned in place with NaOH. The option for automated cleaning in place is available on request which includes a specialized program with additional valves. A Steam in Place system option is also available upon request.



The NXT TFF system by PharmNXT Biotech is a system that’s perfect for running fully automated UF/DF processes at high capacity. It can handle any volume between 20L and 500L (200 g to 2.5 kg), and the compact system, has a low hold-up volume and the capacity to accommodate cassettes with surface area ranging from 0.5 to 2.5 m2

Chromatography Resins (Purolite)

The purification of commercially-available monoclonal antibodies (mAbs) on the market today is typically performed in three chromatography steps. Purolite offer industry-leading solutions for each step. 
Protein A Affinity Chromatography
A Protein A affinity resin is utilized as an initial antibody capture step. Praesto Protein A resins deliver exceptionally high purity (>99%) and yield in a single, efficient step.
Cation Exchange Chromatography
A Sulphopropyl- (SP) functionalized cation exchange resin in ‘bind and elute’ mode is used to remove aggregates and HCP (Host Cell Proteins).
Anion Exchange Chromatography
A Quarternary Amine (Q) functionalized anion exchange resin is used in ‘flow-through’ mode to remove trace contaminates and ensure sufficient viral clearance.


Single-use technology continues to be adopted for many areas of bioprocessing. The use of automation provides additional benefits in manufacturing such as consistency in product quality, reduced labor costs, and reduction of operator errors.

For further process consultancy or sales and service related queries